fgbio

Tools for working with genomic and high throughput sequencing data.

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HardMaskFasta

Overview

Group: FASTA

Converts soft-masked sequence to hard-masked in a FASTA file. All lower case bases are converted to Ns, all other bases are left unchanged. Line lengths are also standardized to allow easy indexing with samtools faidx

Arguments

Name Flag Type Description Required? Max # of Values Default Value(s)
input i PathToFasta Input FASTA file. Required 1  
output o PathToFasta Output FASTA file. Required 1  
line-length l Int Line length or sequence lines. Optional 1 100